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cell line ( homo sapiens ) , sum149  (BioIVT Inc)

 
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    BioIVT Inc cell line ( homo sapiens ) , sum149
    ( a ) Expression of DDI2 in the CRISPR-generated clones of HAP1 cells used in this work was analyzed by western blot. ( b ) The experimental setup used in this study. Cells were pulse treated with bortezomib (Btz) or carfilzomib (Cfz) for 1 hr, then cultured in drug-free media for times indicated and analyzed as described. ( c ) The viability of wt- and DDI2 KO clones of HAP1 cells was measured using CellTiter-Glo, and the inhibition of β5 sites was measured with the Proteasome-Glo assay at times indicated; n=2–5. ( d ) Knockout of DDI2 inhibits the Nrf1 processing. Western blots of Btz-treated HAP1 cells. The sample in the first lane is wt cells treated with VCP/p97 inhibitor CB-5083 immediately after removal of Btz. VCP inhibitors blocks Nrf1 processing ( ; ; ). ( e ) MDA-MB-231 and <t>SUM149</t> cells were analyzed by western blot 72 hr after transfection with DDI2 siRNAs ( f ) Theβ5 activity in siRNA-transfected SUM149 and MDA-MB-231 was measured using Suc-LLVY-AMC immediately and 18 hr after treatment with 100 nM Btz; n=3. ( g ) β5 activity was measured in HCT-116 cells with the Proteasome-Glo assay immediately and 18 hr after treatment with PIs; n=2. Figure 1—source data 1. PDF file containing and original full-size western blot membranes (anti-DDI2, anti-GAPDH) with molecular weight markers. Figure 1—source data 2. Excel file containing data for . Figure 1—source data 3. PDF file containing and original full-size western blot membranes (anti-Nrf1, anti-DDI2, anti-β-actin) with molecular weight markers. Figure 1—source data 4. PDF file containing and full-size western blot membranes (anti-DDI2, anti-β-actin). Additional lanes demonstrate that the knockdown of DDI2 is maintained throughout the experiment. Figure 1—source data 5. Excel file containing data and statistical analysis for . Figure 1—source data 6. Excel file containing data for .
    Cell Line ( Homo Sapiens ) , Sum149, supplied by BioIVT Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+line+(+homo+sapiens+)+%2C+sum149/sum149/pmc11018354-5-7-9
    Average 90 stars, based on 1 article reviews
    cell line ( homo sapiens ) , sum149 - by Bioz Stars, 2026-09
    90/100 stars

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    1) Product Images from "Early recovery of proteasome activity in cells pulse-treated with proteasome inhibitors is independent of DDI2"

    Article Title: Early recovery of proteasome activity in cells pulse-treated with proteasome inhibitors is independent of DDI2

    Journal: eLife

    doi: 10.7554/eLife.91678

    ( a ) Expression of DDI2 in the CRISPR-generated clones of HAP1 cells used in this work was analyzed by western blot. ( b ) The experimental setup used in this study. Cells were pulse treated with bortezomib (Btz) or carfilzomib (Cfz) for 1 hr, then cultured in drug-free media for times indicated and analyzed as described. ( c ) The viability of wt- and DDI2 KO clones of HAP1 cells was measured using CellTiter-Glo, and the inhibition of β5 sites was measured with the Proteasome-Glo assay at times indicated; n=2–5. ( d ) Knockout of DDI2 inhibits the Nrf1 processing. Western blots of Btz-treated HAP1 cells. The sample in the first lane is wt cells treated with VCP/p97 inhibitor CB-5083 immediately after removal of Btz. VCP inhibitors blocks Nrf1 processing ( ; ; ). ( e ) MDA-MB-231 and SUM149 cells were analyzed by western blot 72 hr after transfection with DDI2 siRNAs ( f ) Theβ5 activity in siRNA-transfected SUM149 and MDA-MB-231 was measured using Suc-LLVY-AMC immediately and 18 hr after treatment with 100 nM Btz; n=3. ( g ) β5 activity was measured in HCT-116 cells with the Proteasome-Glo assay immediately and 18 hr after treatment with PIs; n=2. Figure 1—source data 1. PDF file containing and original full-size western blot membranes (anti-DDI2, anti-GAPDH) with molecular weight markers. Figure 1—source data 2. Excel file containing data for . Figure 1—source data 3. PDF file containing and original full-size western blot membranes (anti-Nrf1, anti-DDI2, anti-β-actin) with molecular weight markers. Figure 1—source data 4. PDF file containing and full-size western blot membranes (anti-DDI2, anti-β-actin). Additional lanes demonstrate that the knockdown of DDI2 is maintained throughout the experiment. Figure 1—source data 5. Excel file containing data and statistical analysis for . Figure 1—source data 6. Excel file containing data for .
    Figure Legend Snippet: ( a ) Expression of DDI2 in the CRISPR-generated clones of HAP1 cells used in this work was analyzed by western blot. ( b ) The experimental setup used in this study. Cells were pulse treated with bortezomib (Btz) or carfilzomib (Cfz) for 1 hr, then cultured in drug-free media for times indicated and analyzed as described. ( c ) The viability of wt- and DDI2 KO clones of HAP1 cells was measured using CellTiter-Glo, and the inhibition of β5 sites was measured with the Proteasome-Glo assay at times indicated; n=2–5. ( d ) Knockout of DDI2 inhibits the Nrf1 processing. Western blots of Btz-treated HAP1 cells. The sample in the first lane is wt cells treated with VCP/p97 inhibitor CB-5083 immediately after removal of Btz. VCP inhibitors blocks Nrf1 processing ( ; ; ). ( e ) MDA-MB-231 and SUM149 cells were analyzed by western blot 72 hr after transfection with DDI2 siRNAs ( f ) Theβ5 activity in siRNA-transfected SUM149 and MDA-MB-231 was measured using Suc-LLVY-AMC immediately and 18 hr after treatment with 100 nM Btz; n=3. ( g ) β5 activity was measured in HCT-116 cells with the Proteasome-Glo assay immediately and 18 hr after treatment with PIs; n=2. Figure 1—source data 1. PDF file containing and original full-size western blot membranes (anti-DDI2, anti-GAPDH) with molecular weight markers. Figure 1—source data 2. Excel file containing data for . Figure 1—source data 3. PDF file containing and original full-size western blot membranes (anti-Nrf1, anti-DDI2, anti-β-actin) with molecular weight markers. Figure 1—source data 4. PDF file containing and full-size western blot membranes (anti-DDI2, anti-β-actin). Additional lanes demonstrate that the knockdown of DDI2 is maintained throughout the experiment. Figure 1—source data 5. Excel file containing data and statistical analysis for . Figure 1—source data 6. Excel file containing data for .

    Techniques Used: Expressing, CRISPR, Generated, Clone Assay, Western Blot, Cell Culture, Inhibition, Glo Assay, Knock-Out, Transfection, Activity Assay, Molecular Weight, Knockdown

    ( a ) Cells were treated with PIs for 1 hr, media was shaken off, and cells were cultured in an inhibitor-free fresh media for 48 hr when Alamar Blue assay was performed; n=3–4. See Figure 1 in for a comparison of SUM149 and MDA-MB-231 cells. ( b ) The β5 proteasome activity was measured using Suc-LLVY-AMC in the cell extracts of untreated cells; n=2-8. Figure 1—figure supplement 2—source data 1. Excel file containing data for both panels.
    Figure Legend Snippet: ( a ) Cells were treated with PIs for 1 hr, media was shaken off, and cells were cultured in an inhibitor-free fresh media for 48 hr when Alamar Blue assay was performed; n=3–4. See Figure 1 in for a comparison of SUM149 and MDA-MB-231 cells. ( b ) The β5 proteasome activity was measured using Suc-LLVY-AMC in the cell extracts of untreated cells; n=2-8. Figure 1—figure supplement 2—source data 1. Excel file containing data for both panels.

    Techniques Used: Cell Culture, Alamar Blue Assay, Comparison, Activity Assay


    Figure Legend Snippet:

    Techniques Used: Generated, CRISPR, Mutagenesis, Transfection, Construct, Sequencing, Bradford Assay, Isolation, Reverse Transcription, SYBR Green Assay, Viability Assay, Software



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    cell line ( homo sapiens ) , sum149  (BioIVT Inc)
    90
    BioIVT Inc cell line ( homo sapiens ) , sum149
    ( a ) Expression of DDI2 in the CRISPR-generated clones of HAP1 cells used in this work was analyzed by western blot. ( b ) The experimental setup used in this study. Cells were pulse treated with bortezomib (Btz) or carfilzomib (Cfz) for 1 hr, then cultured in drug-free media for times indicated and analyzed as described. ( c ) The viability of wt- and DDI2 KO clones of HAP1 cells was measured using CellTiter-Glo, and the inhibition of β5 sites was measured with the Proteasome-Glo assay at times indicated; n=2–5. ( d ) Knockout of DDI2 inhibits the Nrf1 processing. Western blots of Btz-treated HAP1 cells. The sample in the first lane is wt cells treated with VCP/p97 inhibitor CB-5083 immediately after removal of Btz. VCP inhibitors blocks Nrf1 processing ( ; ; ). ( e ) MDA-MB-231 and <t>SUM149</t> cells were analyzed by western blot 72 hr after transfection with DDI2 siRNAs ( f ) Theβ5 activity in siRNA-transfected SUM149 and MDA-MB-231 was measured using Suc-LLVY-AMC immediately and 18 hr after treatment with 100 nM Btz; n=3. ( g ) β5 activity was measured in HCT-116 cells with the Proteasome-Glo assay immediately and 18 hr after treatment with PIs; n=2. Figure 1—source data 1. PDF file containing and original full-size western blot membranes (anti-DDI2, anti-GAPDH) with molecular weight markers. Figure 1—source data 2. Excel file containing data for . Figure 1—source data 3. PDF file containing and original full-size western blot membranes (anti-Nrf1, anti-DDI2, anti-β-actin) with molecular weight markers. Figure 1—source data 4. PDF file containing and full-size western blot membranes (anti-DDI2, anti-β-actin). Additional lanes demonstrate that the knockdown of DDI2 is maintained throughout the experiment. Figure 1—source data 5. Excel file containing data and statistical analysis for . Figure 1—source data 6. Excel file containing data for .
    Cell Line ( Homo Sapiens ) , Sum149, supplied by BioIVT Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+line+(+homo+sapiens+)+%2C+sum149/sum149/pmc11018354-5-7-9
    Average 90 stars, based on 1 article reviews
    cell line ( homo sapiens ) , sum149 - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

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    ( a ) Expression of DDI2 in the CRISPR-generated clones of HAP1 cells used in this work was analyzed by western blot. ( b ) The experimental setup used in this study. Cells were pulse treated with bortezomib (Btz) or carfilzomib (Cfz) for 1 hr, then cultured in drug-free media for times indicated and analyzed as described. ( c ) The viability of wt- and DDI2 KO clones of HAP1 cells was measured using CellTiter-Glo, and the inhibition of β5 sites was measured with the Proteasome-Glo assay at times indicated; n=2–5. ( d ) Knockout of DDI2 inhibits the Nrf1 processing. Western blots of Btz-treated HAP1 cells. The sample in the first lane is wt cells treated with VCP/p97 inhibitor CB-5083 immediately after removal of Btz. VCP inhibitors blocks Nrf1 processing ( ; ; ). ( e ) MDA-MB-231 and SUM149 cells were analyzed by western blot 72 hr after transfection with DDI2 siRNAs ( f ) Theβ5 activity in siRNA-transfected SUM149 and MDA-MB-231 was measured using Suc-LLVY-AMC immediately and 18 hr after treatment with 100 nM Btz; n=3. ( g ) β5 activity was measured in HCT-116 cells with the Proteasome-Glo assay immediately and 18 hr after treatment with PIs; n=2. Figure 1—source data 1. PDF file containing and original full-size western blot membranes (anti-DDI2, anti-GAPDH) with molecular weight markers. Figure 1—source data 2. Excel file containing data for . Figure 1—source data 3. PDF file containing and original full-size western blot membranes (anti-Nrf1, anti-DDI2, anti-β-actin) with molecular weight markers. Figure 1—source data 4. PDF file containing and full-size western blot membranes (anti-DDI2, anti-β-actin). Additional lanes demonstrate that the knockdown of DDI2 is maintained throughout the experiment. Figure 1—source data 5. Excel file containing data and statistical analysis for . Figure 1—source data 6. Excel file containing data for .

    Journal: eLife

    Article Title: Early recovery of proteasome activity in cells pulse-treated with proteasome inhibitors is independent of DDI2

    doi: 10.7554/eLife.91678

    Figure Lengend Snippet: ( a ) Expression of DDI2 in the CRISPR-generated clones of HAP1 cells used in this work was analyzed by western blot. ( b ) The experimental setup used in this study. Cells were pulse treated with bortezomib (Btz) or carfilzomib (Cfz) for 1 hr, then cultured in drug-free media for times indicated and analyzed as described. ( c ) The viability of wt- and DDI2 KO clones of HAP1 cells was measured using CellTiter-Glo, and the inhibition of β5 sites was measured with the Proteasome-Glo assay at times indicated; n=2–5. ( d ) Knockout of DDI2 inhibits the Nrf1 processing. Western blots of Btz-treated HAP1 cells. The sample in the first lane is wt cells treated with VCP/p97 inhibitor CB-5083 immediately after removal of Btz. VCP inhibitors blocks Nrf1 processing ( ; ; ). ( e ) MDA-MB-231 and SUM149 cells were analyzed by western blot 72 hr after transfection with DDI2 siRNAs ( f ) Theβ5 activity in siRNA-transfected SUM149 and MDA-MB-231 was measured using Suc-LLVY-AMC immediately and 18 hr after treatment with 100 nM Btz; n=3. ( g ) β5 activity was measured in HCT-116 cells with the Proteasome-Glo assay immediately and 18 hr after treatment with PIs; n=2. Figure 1—source data 1. PDF file containing and original full-size western blot membranes (anti-DDI2, anti-GAPDH) with molecular weight markers. Figure 1—source data 2. Excel file containing data for . Figure 1—source data 3. PDF file containing and original full-size western blot membranes (anti-Nrf1, anti-DDI2, anti-β-actin) with molecular weight markers. Figure 1—source data 4. PDF file containing and full-size western blot membranes (anti-DDI2, anti-β-actin). Additional lanes demonstrate that the knockdown of DDI2 is maintained throughout the experiment. Figure 1—source data 5. Excel file containing data and statistical analysis for . Figure 1—source data 6. Excel file containing data for .

    Article Snippet: Cell line ( Homo sapiens ) , SUM149 , BioIVT , RRID: CVCL_3422 , .

    Techniques: Expressing, CRISPR, Generated, Clone Assay, Western Blot, Cell Culture, Inhibition, Glo Assay, Knock-Out, Transfection, Activity Assay, Molecular Weight, Knockdown

    ( a ) Cells were treated with PIs for 1 hr, media was shaken off, and cells were cultured in an inhibitor-free fresh media for 48 hr when Alamar Blue assay was performed; n=3–4. See Figure 1 in for a comparison of SUM149 and MDA-MB-231 cells. ( b ) The β5 proteasome activity was measured using Suc-LLVY-AMC in the cell extracts of untreated cells; n=2-8. Figure 1—figure supplement 2—source data 1. Excel file containing data for both panels.

    Journal: eLife

    Article Title: Early recovery of proteasome activity in cells pulse-treated with proteasome inhibitors is independent of DDI2

    doi: 10.7554/eLife.91678

    Figure Lengend Snippet: ( a ) Cells were treated with PIs for 1 hr, media was shaken off, and cells were cultured in an inhibitor-free fresh media for 48 hr when Alamar Blue assay was performed; n=3–4. See Figure 1 in for a comparison of SUM149 and MDA-MB-231 cells. ( b ) The β5 proteasome activity was measured using Suc-LLVY-AMC in the cell extracts of untreated cells; n=2-8. Figure 1—figure supplement 2—source data 1. Excel file containing data for both panels.

    Article Snippet: Cell line ( Homo sapiens ) , SUM149 , BioIVT , RRID: CVCL_3422 , .

    Techniques: Cell Culture, Alamar Blue Assay, Comparison, Activity Assay

    Journal: eLife

    Article Title: Early recovery of proteasome activity in cells pulse-treated with proteasome inhibitors is independent of DDI2

    doi: 10.7554/eLife.91678

    Figure Lengend Snippet:

    Article Snippet: Cell line ( Homo sapiens ) , SUM149 , BioIVT , RRID: CVCL_3422 , .

    Techniques: Generated, CRISPR, Mutagenesis, Transfection, Construct, Sequencing, Bradford Assay, Isolation, Reverse Transcription, SYBR Green Assay, Viability Assay, Software